Effects of Different Hydrolysis Methods on the Hydrolysate Characteristics and Photo-Fermentative Hydrogen Production Performance of Corn and Sorghum Straw

The effects of hydrolysis methods (hydrothermal, acid, alkali, hydrothermal-enzyme, acid-enzyme, and alkali-enzyme) on hydrolysate characteristics photo fermentative hydrogen production (PFHP) corn straw (CS) sorghum (SS) were investigated. optimum reducing the sugar in different solvent environments was studied by one-step co-enzymatic pretreatment through a 3,5-dinitrosalicylic acid method. process photolytic fermentation hydrolysates Rhodobacter sphaeroides HY01 further analyzed gas chromatograph, including differences accumulated PFHP yield, chemical oxygen consumption (COD), volatile fatty (VFA) composition. results showed that highest yield obtained method among hydrolysis. In contrast, acid-enzyme can increase which reached 0.42 g·g−1-straw both straws. Both CS SS had from hydrolysate, 122.72 ± 3.34 mL·g−1-total solid (TS) 170.04 4.12 mL·g−1-TS, respectively, compared with their 40.46% 10.53% higher yields, respectively. use enzymatic significantly for to SS, indicating more suitable CS.